EZ-Link Sulfo NHS-SS Biotinylation Kit/EZ-Link Sulfo NHS-SS生物素化试剂盒
货号:21445
规格:10 reactions
价格:7892
产品类型:蛋白标记
品牌:Thermo Fisher
The Thermo Scientific EZ-Link Sulfo NHS-SS Biotinylation Kit contains sufficient reagents for 10 biotinylation reactions (e.g., 1–10 mg antibody per reaction). The EZ-Link Sulfo-NHS-SS-Biotin included in the kit is a water-soluble, NHS-ester biotinylation reagent whose spacer arm includes a cleavable disulfide bond for reversible labeling of proteins and cell surface primary amines.Sulfo-NHS-SS-Biotin is a thiol-cleavable amine-reactive biotinylation reagent that contains an extended spacer arm to reduce steric hindrances associated with avidin binding. This reagent is water-soluble, enabling biotinylation to be performed in the absence of organic solvents such as DMSO or DMF for applications that cannot tolerate solvents or are complicated by their inclusion. The compound is particularly useful for labeling and purifying cell surface proteins, because (a) its sulfonate group prevents it from permeating cell membranes and (b) its cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns.We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency, and performance for the intended research applications.N-Hydroxysulfosuccinimide (NHS) estersof biotin are the most popular type of biotinylation reagent. NHS-activated biotins react efficiently with primary amino groups (-NH2) in alkaline buffers to form stable amide bonds. Proteins (e.g., antibodies) typically have several primary amines that are available as targets for labeling, including the side chain of lysine (K) residues and the N-terminus of each polypeptide.Varieties of biotinNHS-ester reagents differ in length, solubility, cell permeability and cleavability. Non-sulfonated NHS-biotins are cell permeable but must be dissolved in organic solvent such as DMSO or DMF. Sulfo-NHS biotins (and those with pegylated spacers) are directly water soluble but not membrane permeable. Varieties containing disulfide bonds can be cleaved using reducing agents, enabling the biotin group to be disconnected from the labeled protein. |
特点 |
•Protein labeling– biotinylate antibodies to facilitate immobilization, purification or detection•Cell surface labeling– biotinylates only surface proteins of whole cells because the negatively charged reagent does not permeate cell membranes•Amine-reactive—reacts with primary amines (-NH2), such as lysine side-chains, or the amino-termini of polypeptides•Cleavable—disulfide bond in spacer arm allows the biotin label to be removed using reducing agents such as DTT; only a small sulfhydryl group remains attached to the molecule•Soluble– charged sulfo-NHS group increases reagent water solubility compared to ordinary NHS-ester compounds•Medium length—spacer arm (total length added to target) is 24.3 angstroms; it consists of the native biotin valeric acid group extended by a 7-atom chain |
数据 |
Properties of Sulfo-NHS-SS-BiotinThis amine-reactive compound has a cleavable disulfide bond and is useful for cell surface protein labeling and isolation. |
技术参数 组成成分 Sufficient For: 10 labeling reactions, each with 1 to 10 mg of antibody• EZ-Link Sulfo-NHS-SS-Biotin, 25 mg• PBS Pack (makes 500 mL), 1 pack• Zeba Spin Desalting Columns (7K MWCO), 5 mL, 10 columns• HABA Solution, 1 mL• Avidin, 10 mg
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://biorod.cn/thread-26536.htm