DyLight 405 Maleimide/DyLight 405马来酰亚胺
货号：46600
规格：1 mg
价格：4326
产品类型：蛋白标记
品牌：Thermo Fisher
| Thermo Scientific DyLight 405 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 405 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.DyLight 405has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Alexa Fluor 405, Cascade Blue and AMCA dyes in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.DyLight 405 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more aboutmaleimide chemistry. |
| 特点 |
| •High performance—DyLight 405 shows brighter fluorescence than Alexa Fluor 405, Cascade Blue and AMCA•Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)•Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling•Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency |
| 应用 |
| • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies |
| 数据 |
Immunofluorescence microscopy using DyLight 405 dyeVimentin (red) and lamin B1 (blue) in normal human colon tissue were fluorescently labeled with specific primary antibodies and DyLight 405-Conjugated Goat Anti-Mouse or DyLight 549-Conjugated Goat Anti-Rabbit Secondary Antibodies, respectively. Cell nuclei were counterstained green. |
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