DyLight 755 Microscale Antibody Labeling Kit/DyLight 755微型抗体标记试剂盒
货号：84539
规格：5 reactions
价格：5692
产品类型：蛋白标记
品牌：Thermo Fisher
| The Thermo Scientific DyLight 755 Microscale Antibody Labeling Kit contains an NHS ester-activated derivative of high-performance DyLight 755 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100 µg of IgG—the amine-reactive DyLight 755 NHS-ester in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.DyLight 755is a near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight 755 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor 750. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight 755 Amine-Reactive Dye is also available as a stand-alone reagent.DyLight 755 Amine-Reactive Dye is activated with anN-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more aboutNHS ester chemistry.Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 755 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling. |
| 特点 |
| •High performance—DyLight 755 replaces Alexa Fluor 755 for near-infrared staining•Specific—NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)•Convenient kit sizes—standard and microscale sizes are offered to match your experimental needs•Optimized procedure—following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling |
| 应用 |
| • Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting, or ELISA assay• Target protein labeling for in vitro and in vivo fluorescent detection strategies |
| 数据 |
DyLight Antibody Labeling Kits provide outstanding recoveryThe percent recovery for DyLight Antibody Labeling Kits is the average for 16 labeling reactions using three different antibodies. The percent recovery for DyLight Microscale Antibody Labeling Kits is the average for 13 labeling reactions using three different antibodies. Infrared Western blot detection of ERK1 using DyLight 755-conjugated secondary antibodySerial dilutions of A562 cell lysates were analyzed by Western blotting for levels of ERK1 using a mouse anti-ERK1 primary antibody and a goat anti-mouse secondary antibody conjugated to DyLight 755 (using the DyLight 755 Microscale Antibody Labeling Kit). Left lane: Infrared molecular weight marker. Procedure summary for DyLight 350 Antibody Labeling KitsThe protocol is simple and provides efficient labeling, exceptional clean-up and excellent antibody recovery. |
技术参数 组成成分 Sufficient For: Labeling and purifying 5 x 100 µg (1 mg/mL) of IgG or other proteins• DyLight Fluor NHS Ester, 5 x 15 µg vials• Borate Buffer (0.67M), 1 mL• Purification Resin, 5 mL• Microcentrifuge Spin Columns, 5 columns• Microcentrifuge Collection Tubes, 10 tubes
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