Fluorescein-5-Maleimide/荧光素5-马来酰亚胺
货号：62245
规格：25 mg
价格：4096
产品类型：蛋白标记
品牌：Thermo Fisher
| Thermo Scientific Fluorescein-5-Maleimide is a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes. Fluorescein-5-Maleimide is a sulfhydryl-reactive derivative of fluorescein that labels proteins and other molecules having free thiols (cysteine side chains) |
| 特点 |
| • Alternative names: 5-MF, 5-maleimido-fluorescein• Chemical name: 1H-Pyrrole-2,5-dione, 1-(3',6'-dihydroxy-3-oxospiro (isobenzofuran-1(3H),9'-(9H)xanthen-5-yl)-• Molecular weight: 427.36±3• Excitation source: 488 nm spectral line, argon-ion laser• Excitation wavelength: 494 nm• Emission wavelength: 518 nm• Extinction coefficient: > 80,000 M-1cm-1• CAS #: 75350-46-8• Solubility: Soluble in DMF or DMSO• Reactive groups: Maleimide, reacts with sulfhydryls at pH 6.5 to 7.5 |
| 应用 |
| • Label antibodies for use as immunofluorescent probes• Label oligonucleotides for hybridization probes• Detect proteins in gels and on Western blots |
| 数据 |
Detection of α-tubulin in A549 cells demonstrates use of fluorescein-labeled secondary antibodyCells were grown in 96-well microplates for 18-20 hrs, fixed with 4% paraformaldehyde (Part No. 28906) and permeabilized with 0.1% Surfact-Amps X-100 (Part No. 28314). Cells were then probed with a mouse anti-α-tubulin primary antibody (0.4µg/mL) and Fluorescein-goat anti-mouse secondary antibody (2µg/mL). Nuclei were labeled with Hoechst Dye. Images were acquired by fluorescence microscopy.A.Fluorescence image shows a delicate network of α-tubulin (pseudo-colored green) located exclusively in the cytoplasm.B.Nuclear counterstain with Hoechst Dye (pseudo-colored blue)C.Merged image. Chemical structures of Fluorescein-5-maleimide and 5-IAFBoth of these compounds allow fluorescent labeling of sulfhydryl groups on proteins and other molecules. |
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