CD127 Monoclonal Antibody (A7R34), Functional Grade, eBioscience™/CD127 单克隆 (A7R34), 功能抗体, eBioscience™
货号：16-1271-85,16-1271-025,16-1271-050
规格：500 µg,25 mg,50 mg
价格：3735,72266,120443
产品类型：流式抗体
品牌：eBioscience
抗原：CD127
物种：人/小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：A7R34
荧光染料：其它
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类型: | 一抗(功能抗体） | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Functional Grade, eBioscience |
浓度： | 1 mg/mL | 用法： | 0.5 µg/test(Flow);Assay-Dependent（中和）;Assay-Dependent（功能检测） |
产品详细信息Description: The A7R34 monoclonal antibody reacts with mouse CD127, the high affinity alpha subunit of the mouse IL-7 receptor. IL-7 receptor alpha chain is expressed by immature B cells in the bone marrow, double-negative (CD4-CD8-), single-positive (CD4+ and CD8+), but not double-positive (CD4+CD8+) thymocytes. In the periphery, mature T cells express CD127 at low level. A7R34 inhibits binding of IL-7 to its receptor and has been used in in vivo and in vitro studies to elucidate the role of IL-7 in T and B cell development and activation. Binding of A7R34 blocks the binding of SB/199, another antibody which recognizes mouse CD127.Applications Reported: The A7R34 antibody has been reported for use in flow cytometric analysis. It has also been reported in blocking of IL-7 binding in functional studies.Applications Tested: The A7R34 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.靶标信息CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.仅用于科研。不用于诊断过程。
CD127 Antibody (16-1271-85) in FlowStaining of C57Bl/6 splenocytes with 0.5 µg of Rat IgG2a K Isotype Control Functional Grade Purified (Product # 16-4321-82) (open histogram) or 0.25 µg of Anti-Mouse CD127 Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87).Cells in the lymphocyte gate were used for analysis.
二抗
荧光二抗
免疫组化一抗
标签抗体
流式
1.HaematologicaBone marrow adipose tissue-derived stem cell factor mediates metabolic regulation of hematopoiesis."Published figure using CD127 monoclonal antibody (Product # 16-1271-85) in Flow Cytometry"
2.ImmunityAn Id2RFP-Reporter Mouse Redefines Innate Lymphoid Cell Precursor Potentials."Published figure using CD127 monoclonal antibody (Product # 16-1271-85) in Flow Cytometry"
IF
3.ImmunityRank signaling links the development of invariant γδ T cell progenitors and Aire(+) medullary epithelium."16-1271 was used in Immunofluorescence to study the temporal development of Vγ5(+) γδ T cells during thymus medulla formation."AuthorsRoberts NA,White AJ,Jenkinson WE,Turchinovich G,Nakamura K,Withers DR,McConnell FM,Desanti GE,Benezech C,Parnell SM,Cunningham AF,Paolino M,Penninger JM,Simon AK,Nitta T,Ohigashi I,Takahama Y,Caamano JH,Hayday AC,Lane PJ,Jenkinson EJ,Anderson G4.Journal of immunology (Baltimore, Md. : 1950)LTbetaR signaling induces cytokine expression and up-regulates lymphangiogenic factors in lymph node anlagen.AuthorsVondenhoff MF,Greuter M,Goverse G,Elewaut D,Dewint P,Ware CF,Hoorweg K,Kraal G,Mebius RE
QQ:1749072012