Alexa Fluor 405 NHS Ester (Succinimidyl Ester)/Alexa Fluor 405 NHS酯(琥珀酰亚胺酯)
货号:A30100,A30000
规格:5 mg,1 mg
价格:23512,6093
产品类型:蛋白标记
品牌:Thermo Fisher
Alexa Fluor 405 is a blue-fluorescent dye optimal for use with the 405 nm violet laser. Used for stable signal generation in imaging and flow cytometry, Alexa Fluor 405 dye is water soluble and pH-insensitive from pH 4 to pH 10. In addition to reactive dye formulations, we offer Alexa Fluor 405 dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection (learn more).The NHS ester (or succinimidyl ester) of Alexa Fluor 405 is the most popular tool for conjugating this dye to a protein or antibody. NHS esters can be used to label to the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting Alexa Fluor conjugate will exhibit brighter fluorescence and greater photostability than the conjugates of other spectrally similar fluorophores.Typical Conjugation ReactionYou can conjugate amine-reactive reagents with virtually any protein or peptide (the provided protocol is optimized for IgG antibodies). You can scale the reaction for any amount of protein, but the concentration of the protein should be at least 2 mg/mL for optimal results. We recommend trying three different degrees of labeling, using three different molar ratios of the reactive reagent to protein.The Alexa Fluor NHS ester is typically dissolved in high-quality anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO) (D12345), and the reaction is carried out in 0.1–0.2 M sodium bicarbonate buffer, pH 8.3, at room temperature for 1 hour. Because the pKa of the terminal amine is lower than that of the lysine epsilon-amino group, you may achieve more selective labeling of the amine terminus using a buffer closer to neutral pH.Conjugate PurificationLabeled antibodies are typically separated from free Alexa Fluor dye using a gel filtration column, such as Sephadex G-25, BioGel P-30, or equivalent. For much larger or smaller proteins, select a gel filtration media with an appropriate molecular weight cut-off or purify by dialysis. We offer several purification kits optimized for different quantities of antibody conjugate:Antibody Conjugate Purification Kit for 0.5-1 mg (A33086)Antibody Conjugate Purification Kit for 20-50 µg (A33087)Antibody Conjugate Purification kit for 50-100 µg (A33088)Learn More About Protein and Antibody LabelingWe offer a wide selection of Molecular Probes antibody and protein labeling kits to fit your starting material and your experimental setup. See ourAntibody Labeling kitsor use ourLabeling Chemistry Selection Toolfor other choices. To learn more about our labeling kits, readKits for Labeling Proteins and Nucleic Acids—Section 1.2in The Molecular Probes Handbook.We’ll Make a Custom Conjugate for YouIf you can’t find what you’re looking for in our online catalog, we’ll prepare a custom antibody or protein conjugate for you. Ourcustom conjugation serviceis efficient and confidential, and we stand by the quality of our work. We are ISO 9001:2000 certified.
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价,请加客服QQ:1749072012 、客服微信:jinshanbio,或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务,数据资源基于CC协议。
© 本文地址:
https://biorod.cn/thread-26635.htm