• 标记抗体可作为免疫荧光探针• 用于杂交探针的标记寡核苷酸• 检测凝胶和 Western 印迹中的蛋白
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Chemical structures of Fluorescein-5-maleimide and 5-IAFBoth of these compounds allow fluorescent labeling of sulfhydryl groups on proteins and other molecules.
Detection of α-tubulin in A549 cells demonstrates use of fluorescein-labeled secondary antibodyCells were grown in 96-well microplates for 18-20 hrs, fixed with 4% paraformaldehyde (Part No. 28906) and permeabilized with 0.1% Surfact-Amps X-100 (Part No. 28314). Cells were then probed with a mouse anti-α-tubulin primary antibody (0.4µg/mL) and Fluorescein-goat anti-mouse secondary antibody (2µg/mL). Nuclei were labeled with Hoechst Dye. Images were acquired by fluorescence microscopy.A.Fluorescence image shows a delicate network of α-tubulin (pseudo-colored green) located exclusively in the cytoplasm.B.Nuclear counterstain with Hoechst Dye (pseudo-colored blue)C.Merged image.
QQ:1749072012