Hydrazide-AMCA (hydrazide-activated aminomethylcoumarin acetate)/酰肼-AMCA（酰肼活化的氨基甲基香豆素乙酸酯）
货号：33015
规格：5 mg
价格：0
产品类型：蛋白标记
品牌：Thermo Fisher
| AMCA (aminomethylcoumarin acetate) is a blue fluorescent dye with reactive derivatives that are used as contrasting probes for double- and triple-labeling in immunofluorescence microscopy, arrays and in situ hybridization. The desirable properties of AMCA dyes include a relatively large Stoke's shift and resistance to photobleaching. NHS-AMCA and Sulfo-NHS-AMCA are reactive towards primary amine groups on proteins, peptides and other biomolecules. AMCA-Hydrazide is used to label glycosylation sites or for conjugation to carboxyl groups using the crosslinker EDC. AMCA has an excitation maximum at 345nm and an emission maximum at 450nm. |
| 特点 |
| •AMCA—activated derivatives of blue-fluorescent aminomethylcoumarin acetate dye•Amine-specific labeling—NHS-ester and Sulfo-NHS-ester varieties of AMCA efficiently label antibodies and other purified proteins at primary amines (lysine side chains)•Glycoprotein labeling—hydrazide variety of AMCA labels oxidized sugar groups in glycoproteins or other carbohydrates |
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