LavaLAMP™ DNA Master Mix with Dye/DNA扩增预混合液含染料
货号：30067-1
规格：200 rxn
价格：5051
产品类型：PCR 及DNA聚合酶
品牌：Lucigen
| The LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP
(loop-mediated isothermal amplification) reactions. LAMP kits are commonly available as multicomponent
kits that require optimization (e.g. MgSO4, betaine, enzyme as well as temperature, primer
concentration, etc.). The LavaLAMP™ DNA Master Mix greatly simplifies reaction optimization by limiting
optimization to target specific components/conditions (LAMP primers, target concentration and reaction
temperature). Additionally, this master mix is heat stable at 90˚C for ≤5 minutes, which enables the
addition of a reaction preheating step to help reduce the time to result (TTR) when using purified target DNA as input. This kit is for research purposes only, and available under the limited-use license
described at the end of this document. LavaLAMP™DNA预混液旨在简化DNA LAMP（环—介导的等温扩增）反应的开发和优化。 LAMP试剂盒通常是需要优化的多组分试剂盒（例如MgSO4，甜菜碱，酶以及温度，引物浓度等）。 LavaLAMP™DNA预混液通过将优化限制在特定目标组分/条件（LAMP引物，目标浓度和反应温度）上，大大简化了反应优化过程。 此外，该预混合物在90°C的温度下热稳定≤5分钟，这使得添加反应预热步骤有助于减少使用纯化的靶DNA作为起始时的生成时间（TTR）。 该试剂盒仅用于研究目的，可根据本文档末尾所述的有限使用许可使用。 |
| 优点： |
| ▪Loop-mediated isothermal amplification (LAMP):Facilitates running amplification reactions in difficult test environments, enabling use outside of a high-tech laboratory；▪Master Mix Format:Simplifies reaction setup and minimizes optimization required to produce the best assay results；▪High Thermal Stability:Highly thermostable enzyme (up to 90°C for 5 min) enables preheating of reactions containing purified target DNA which, depending on the target, may increase assay sensitivity and decrease time to results；▪Elevated LAMP Reaction Temperature (68-74°C):Improves primer specificity and reduces background amplification depending on the DNA target；▪Freeze-dry Compatible:Enables generation of room temperature stable test kits through lyophilization.▪环-介导的等温扩增（LAMP）：便于在困难的测试环境中进行扩增反应，从而可以在高科技实验室之外使用。▪预混液形式：简化反应设置，并最简化产生最佳测定结果所需的优化。▪高热稳定性：高度热稳定的酶（在90°C下持续5分钟）可以预热含有纯化靶DNA的反应，具体取决于靶标，可以提高测定的灵敏度并缩短结果的产生时间。▪升高的LAMP反应温度（68-74°C）：提高引物特异性并减少取决于DNA靶的背景扩增；▪兼容冻干：通过冻干可生成室温稳定的测试试剂盒。 |
| 数据： |
| Figure 1. Simplified overview of the loop-mediated isothermal amplification (LAMP) process.For simplicity, 4/6 potential LAMP primers are illustrated. The inclusion of two additional primers, F-Loop; Forward Loop Primer and B-Loop; Backward Loop Primer, often significantly enhances amplification. For more information, please visit the Eiken website.图1.环路-介导的等温扩增（LAMP）过程的简化概述。 为了简单起见，展示出了4/6个潜在的LAMP引物。 包括两个额外的引物F-Loop； 正向循环引物和B环； 向后循环引物通常可以显着增强扩增。 有关更多信息，请访问Eiken网站。Figure 2. Illustration of the differences in LAMP reaction setup and potential optimization parameters when using a master mix vs. a component kit format.Panel A illustrates the components added to a LAMP reaction (left) when using the LavaLAMP DNA Master Mix and the various parameters than can be optimized (right). Panel B also illlustrates reaction setup (left) and potential optimization parameters (right) when using a standard LAMP component kit图2.使用预混液与组分试剂盒形式时，LAMP反应设置和潜在优化参数的差异说明。 A说明了使用LavaLAMP DNA Master Mix时添加到LAMP反应中的组分（左）以及各种参数（可以优化）（右）。 当使用标准LAMP组件试剂盒时， B还说明了反应设置（左）和潜在的优化参数（右）。Figure 3. Loop-mediated isothermal amplification (LAMP) with real-time fluorescent detection of amplified products.LAMP reactions were set up using the indicated kits according to manufacturer’s recommendations. Target DNA (C. difficile) at varying input amounts, tcdA target LAMP primers, and Green Fluorescent Dye (LavaLAMP Kit) were included in all reactions. Reactions were run on a CFX96 Thermal Cycler (Bio-Rad) at the following temperatures: LavaLAMP; 68°C; other kits at the recommended 65°C and fluorescence was measured over 60 minutes to determine the TTR. NTC = No Target Control图3.环-介导的等温扩增（LAMP），对扩增产物进行实时荧光检测。 根据制造商的建议，使用指定的试剂盒设置LAMP反应。 所有反应中都包括输入量不同的目标DNA（C. difficile），tcdA目标LAMP引物和绿色荧光染料（LavaLAMP试剂盒）。 反应在以下温度下在CFX96热循环仪（Bio-Rad）上进行： 68°C; 其他试剂盒在建议的65°C下进行，并在60分钟内测量荧光，以确定TTR。 NTC =无靶标控制。 |
| 相关产品： |
| ▪LavaLAMP™ DNA Component Kit（#30076）▪Green Fluorescent Dye（#30078） ▪ LavaLAMP™ DNA Master Mix (#30066-1) |
技术参数 产品优点 - Loop-mediated isothermal amplification (LAMP): Facilitates running amplification reactions in difficult test environments, enabling use outside of a high-tech laboratory. - Master Mix Format: Simplifies reaction setup and minimizes optimization required to produce the best assay results. - High Thermal Stability: Highly thermostable enzyme (up to 90°C for 5 min) enables preheating of reactions containing purified target DNA which, depending on the target, may increase assay sensitivity and decrease time to results.- 环-介导的等温扩增（LAMP）：便于在困难的测试环境中进行扩增反应，从而可以在高科技实验室之外使用。- 预混液形式：简化反应设置，并最简化产生最佳测定结果所需的优化。- 高热稳定性：高度热稳定的酶（在90°C下持续5分钟）可以预热含有纯化靶DNA的反应，具体取决于靶标，可以提高测定的灵敏度并缩短结果的产生时间。
产品应用 - The LavaLAMP™ DNA Master Mix is intended to simplify development and optimization of DNA LAMP (loop-mediated isothermal amplification) reactions. - LavaLAMP™DNA预混液旨在简化DNA LAMP（环介导的等温扩增）反应的开发和优化。
组成成分 LavaLAMP™ DNA Master Mix kits contain: LavaLAMP DNA Master Mix, DNA Positive Control LAMP Primer Mix, and DNA Positive Control.
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-18119.htm
QQ:1749072012