DyLight 800 Maleimide/DyLight 800马来酰亚胺
货号：46621
规格：1 mg
价格：4326
产品类型：蛋白标记
品牌：Thermo Fisher
| Thermo Scientific DyLight 800 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 800 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.DyLight 800is a near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight 800 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor 790 and IRDye™ 800. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.DyLight 800 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. |
| 特点 |
| •High performance—DyLight 800 replaces Alexa Fluor 800 and IRDye 800 for near-infrared staining•Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)•Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling•Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency |
| 应用 |
| • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting, and ELISA assay• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies |
| 数据 |
Two-color infrared Western blot detection using DyLight 680- and DyLight 800-Conjugated Secondary Antibodiesp53 (53 kDa; red) and cyclophilin B (21 kDa; green) levels in response to siRNA expression in A549 cells were detected by Western blot analysis using DyLight 680- (red) and DyLight 800- (green) Conjugated Secondary Antibodies. Beta-catenin (90 kDa, green) was used as the load control. Imaged with the Odyssey™ Infrared Imaging System using the 700 and 800 channels. |
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