CD172a (SIRP alpha) Monoclonal Antibody (P84), PE
货号：12-1721-80,12-1721-82
规格：25μg,100μg
价格：1442,3305
产品类型：流式抗体
品牌：Thermo Fisher
物种：小鼠
宿主：大鼠
抗体亚型：IgG1, kappa
克隆号：P84
荧光染料：PE
| 抗体类型 | 单抗 | 同型对照： | Rat IgG1 kappa Isotype Control (eBRG1), PE |
| 浓度： | 0.2 mg/mL | 用法： | 0.5 μg/test (Flow)； |
| 产品详细信息Description: This P84 monoclonal antibody reacts with mouse CD172a, also known as signal regulatory protein a (SIRPa). This cell surface glycoprotein consists of three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The ITIM domains have been demonstrated to recruit and bind the Src homology 2 domain-containing phosphatases SHP-1 and SHP-2. CD172a is expressed on monocytes, macrophages, dendritic cells, but not on T and B lymphocytes. Moreover, neurons and other tissues of the central nervous system have also been shown to express CD172a. The integrin-associated protein CD47 is the extracellular ligand for CD172a. Studies show that CD172a is involved in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis.This monoclonal antibody has been reported to have neutralizing activity.Applications Reported: This P84 antibody has been reported for use in flow cytometric analysis.Applications Tested: This P84 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息SIRP alpha (CD172a, signal-regulatory protein alpha) is a receptor-type transmembrane glycoprotein expressed on cells of myeloid origin, including granulocytes, dendritic cells (DCs), macrophages, mast cells and hematopoietic stem cells. SIRP alpha acts as a substrate for several activated tyrosine kinases, including EGFR, PDGFR, src and insulin receptor and is involved in the negative regulation of receptor tyrosine kinase-coupled signaling pathways. The ligand binding of SIRP alpha to integrin-associated protein CD47 results in tyrosine kinase phosphorylation of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within the cytoplasmic region of SIRP alpha, which mediates the recruitment and activation of the tyrosine phosphatases SHP-1 and SHP-2. Ligation of SIRP alpha with CD47 has been demonstrated in several regulatory processes, including the inhibition of host cell phagocytosis by macrophages and the bi-directional activation of T cells and DCs. SIRP alpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion, and plays a general role in different physiological and pathological processes. Cancer cells highly express CD47, which activates SIRP alpha and inhibits macrophage-mediated destruction of cancerous cell growth |
| 数据 |
| CD172a (SIRP alpha) Antibody (12-1721-82) in FlowStaining of C57Bl/6 bone marrow cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.25 µg of Rat IgG1 K Isotype Control PE (Product # 12-4301-82) (left) or 0.25 µg of Anti-Mouse CD172a (SIRP alpha) PE (right). Cells in the large scatter population were used for analysis. |
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| 参考文献： |
| 1.ImmunologySignal-regulatory protein α from the NOD mouse binds human CD47 with an exceptionally high affinity-- implications for engraftment of human cells."12-1721 was used in Flow cytometry/Cell sorting to compare binding affinities of CD47 for different SIRPα mutants to assess the role of leucocyte surface protein interactions with tumours in cancer development."AuthorsKwong LS,Brown MH,Barclay AN,Hatherley D |
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