| 产品详细信息Description: The M5/114.15.2 monoclonal antibody reacts with the mouse major histocompatibility complex class II, both I-A and I-E subregion-encoded glycoproteins (I-A b, I-A d, I-A q, I-E d, I-E k, not I-A f, I-A k, or I-A s). It detects a polymorphic determinant present on B cells, monocytes, macrophages, dendritic cells, and activated T lymphocytes from mice carrying the H-2 b, H-2 d, H-2 q, H-2 p, H-2 r and H-2 u but not from mice carrying the H-2 s or H-2 f haplotypes. The M5/114 mAb is reported to inhibit I-A-restricted T cell responses of the H-2 b, H-2 d, H-2 q, H-2 u but not H-2 f, H-2 k, or H-2 s haplotypes.Applications Reported: M5/114.15.2 has been reported for use in flow cytometric analysis.Applications Tested: The M5/114.15.2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long |
| MHC Class II (I-A/I-E) Antibody (15-5321-82)Cells 2020 -Figure 6 Administration of rGDF3 to LPS-mice improves cardiac function and promotes cardiac macrophage M2 phenotype. WT mice received recombinant GDF3 protein (I.P., 10mug/kg BW) 12 h prior to LPS injection (I.P., 10mg/kg BW), 12 h later cardiac function was measured by echocardiograph. ( A ) Representative M-mode echocardiography recordings for three groups, and ( B ) left ventricular ejection fraction (EF %), ( C ) fractional shortening (FS %) and ( D ) left ventricular internal dimension at end-systolic (LVIDs) were calculated (*, p < 0.05 vs. Ctl; #, p < 0.05 vs. LPS+B; n = 5). Cardiac cells were stained with antibodies to ( E ) MHC-II, ( G ) CD206, ( I ) F4/80 plus CD11b, ( K ) Ly6C for flow cytometry analysis and their respective quantification results are shown in ( F , H , J , L ). (*, p < 0.05; n = 4). Similar results were obtained in other two independent experiments. |
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