| 产品详细信息Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.Applications Reported: The 53-6.7 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells |
| 1.PLoS pathogensExperimental cerebral malaria pathogenesis--hemodynamics at the blood brain barrier."12-0081 was used in Flow cytometry/Cell sorting to show that significantly more CD8(+) T cells, neutrophils, and macrophages are recruited to postcapillary venules during ECM compared to hyperparasitemia."AuthorsNacer A,Movila A,Sohet F,Girgis NM,Gundra UM,Loke P,Daneman R,Frevert U2.Journal of immunology (Baltimore, Md. : 1950)Cutting edge: intravascular staining redefines lung CD8 T cell responses."12-0081 was used in Immunofluorescence to elucidate T cell trafficking and differentiation within the lung after systemic infection."AuthorsAnderson KG,Sung H,Skon CN,Lefrancois L,Deisinger A,Vezys V,Masopust D3.The American journal of pathologyIL-27 inhibits hyperglycemia and pancreatic islet inflammation induced by streptozotocin in mice."12-0081 was used in Immunofluorescence to evaluate inflammatory cell infiltration to pancreatic islets in wild-type, EBI3(-/-), and WSX-1(-/-) mice treated with streptozotocin."AuthorsFujimoto H,Hirase T,Miyazaki Y,Hara H,Ide-Iwata N,Nishimoto-Hazuku A,Saris CJ,Yoshida H,Node K4.Cancer researchPotent induction of tumor immunity by combining tumor cryoablation with anti-CTLA-4 therapy."12-0081 was used in Flow cytometry/Cell sorting to demonstrate a novel combination therapy for the augmentation of antitumour immunity and metastatic rejection."AuthorsWaitz R,Solomon SB,Petre EN,Trumble AE,Fassò M,Norton L,Allison JP5.International journal of clinical and experimental pathologyBTLA associates with increased Foxp3 expression in CD4(+) T cells in dextran sulfate sodium-induced colitis."12-0081 was used in Immunofluorescence to determine that BTLA may be involved in the control of inflammatory responses through increasing Foxp3 expression in DSS-induced colitis."AuthorsZhang HX,Zhu B,Fu XX,Zeng JC,Zhang JA,Wang WD,Kong B,Xiang WY,Zhong J,Wang CY,Zheng XB,Xu JF |
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