CD8a Monoclonal Antibody (RPA-T8), APC-eFluor 780-Bio-Rad-Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱

CD8a Monoclonal Antibody (RPA-T8), APC-eFluor 780

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CD8a Monoclonal Antibody (RPA-T8), APC-eFluor 780

货号：47-0088-41,47-0088-42

规格：25Tests,100Tests

价格：969,2062

产品类型：流式抗体

品牌：Alfa aesar

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

荧光染料：APC-eFluor 780

抗体类型	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor 780
浓度：	5 µL/Test	用法：	5 μL (0.125 μg)/test(Flow)
产品详细信息Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells
数据
CD8a Antibody (47-0088-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD8a APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.
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参考文献：
1.Journal of interferon and cytokine research : the official journal of the International Society for Interferon and Cytokine ResearchLiposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals."Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry"2.Journal of immunology (Baltimore, Md. : 1950)Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells."Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry"3.Stem cells and developmentImmunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells."Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry"4.Frontiers in immunologySafety and Immunogenicity of Malaria Vectored Vaccines Given with Routine Expanded Program on Immunization Vaccines in Gambian Infants and Neonates: A Randomized Controlled Trial.AuthorsMensah VA,Roetynck S,Kanteh EK,Bowyer G,Ndaw A,Oko F,Bliss CM,Jagne YJ,Cortese R,Nicosia A,Roberts R,D'Alessio F,Leroy O,Faye B,Kampmann B,Cisse B,Bojang K,Gerry S,Viebig NK,Lawrie AM,Clarke E,Imoukhuede EB,Ewer KJ,Hill AVS,Afolabi MO5.Journal of immunology researchThe Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma."47-0088 was used in Flow cytometry/Cell sorting to investigate the role of the NLRP3 inflammasome in multiple myeloma."AuthorsZhao X,Hua M,Yan S,Yu J,Han F,Zhong C,Wang R,Zhang C,Hou M,Ma D

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