CD4 Monoclonal Antibody (OKT4 (OKT-4)), APC-eFluor 780
货号：47-0048-42
规格：100 Tests
价格：2986
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG2b, kappa
荧光染料：APC-eFluor 780
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG2b kappa Isotype Control (eBMG2b), APC-eFluor 780 |
| 浓度： | 5 µL/Test | 用法： | 5 µL (0.125 µg)/test (Flow) |
| 产品详细信息Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (47-0048-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD8a FITC (Product # 11-0088-42) and Mouse IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4732-80) (left) or Anti-Human CD4 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
| 参考文献： |
| 1.Nature communicationsIL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells."Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry"2.Stem cell reportsTargeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis."Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry"3.Neuro-oncologyPD-L1 expression and prognostic impact in glioblastoma."Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry"4.Nature communicationsComplement receptor CD46 co-stimulates optimal human CD8+T cell effector function via fatty acid metabolism."Published figure using CD4 monoclonal antibody (Product # 47-0048-42) in Flow Cytometry"5.AtherosclerosisExvivo culture of human atherosclerotic plaques: A model to study immune cells in atherogenesis."47-0048 was used in Flow cytometry/Cell sorting to establish a new ex vivo model of human atherosclerotic plaques that preserves the main cell types in plaques and the extracellular components in the context of native cytoarchitecture." |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-26863.htm
QQ:1749072012