CD45 Monoclonal Antibody (30-F11), PE-Cyanine5, eBioscience™

货号：15-0451-81,15-0451-82,15-0451-83

规格：50μg,100μg,200μg

价格：1518,2230,3551

产品类型：抗体和ELISA

品牌：Thermo Fisher

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine5
免疫原性：		用法：	0.06 μg/test(Flow)

Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: The 30-F11 antibody has been reported for use in flow cytometric analysis.Applications Tested: The 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD45 Antibody (15-0451-81) in Flow Staining of C57Bl/6 splenocytes with 0.03 µg of PE-Cyanine5 Rat IgG2b Isotype Control (Product # 15-4031-82) (open histogram) or 0.03 µg of Anti-Mouse CD45 PE-Cyanine5 (filled histogram). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Stem cell reportsCardiosphere-Derived Cells Require Endoglin for Paracrine-Mediated Angiogenesis.2017.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer2. Journal of cellular and molecular medicineCytochrome P450 26A1 modulates natural killer cells in mouse early pregnancy.2017.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3. JCI insightIrgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.2017.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer4. Immunology and cell biologyT-cell response to herpes simplex virus-1.2017.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer5. The Journal of clinical investigationA CCR4 antagonist reverses the tumor-promoting microenvironment of renal cancer.2017.By clicking the link above, you will be redirected to the Benchsci website. Disclaimer

技术参数

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