CD8a Monoclonal Antibody (OKT8 (OKT-8)), APC-eFluor 780, eBioscience™
货号：47-0086-42
规格：100 Tests
价格：2738
产品类型：流式抗体
品牌：Thermo Fisher
抗原：CD8a
物种：人
宿主：小鼠
抗体亚型：IgG2a, kappa
克隆号： OKT8 (OKT-8)
荧光染料：APC-eFluor 780
| 抗体类型: | 流式抗体 | 同型对照： | IgG2a, kappa |
| 浓度： | 5 µL/Test | 用法： | 5 µL (0.25 µg)/test |
| Description:The OKT8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alphaalpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells. Preliminary testing indicates that OKT8 and two other mouse anti-human CD8 antibodies (clone RPA-T8, Cat. No.14-0088 and clone HIT8a, Cat. No.14-0089) do not compete with each other for binding to human peripheral blood leukocytes by flow cytometric analysis, suggesting that they do not bind to similar epitopes or block each other by steric hindrance.Applications Reported: This OKT8 (OKT-8) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT8 (OKT-8) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor® 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization. |
| 数据 |
CD8a Antibody (47-0086-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0198-42) and staining buffer (autofluorescence) (left) or Anti-Human CD8a APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1. PloS oneMHC multimer-guided and cell culture-independent isolation of functional T cell receptors from single cells facilitates TCR identification for immunotherapy."Published figure using CD8a monoclonal antibody (Product # 47-0086-42) in Flow Cytometry"2.Frontiers in pharmacologyDisruption of PD-1 Enhanced the Anti-tumor Activity of Chimeric Antigen Receptor T Cells Against Hepatocellular Carcinoma."Published figure using CD8a monoclonal antibody (Product # 47-0086-42) in Flow Cytometry"3.Neuro-oncologyPD-L1 expression and prognostic impact in glioblastoma."Published figure using CD8a monoclonal antibody (Product # 47-0086-42) in Flow Cytometry"4.VaccineDiscordance between antibody and T cell responses in recipients of trivalent inactivated influenza vaccine."Published figure using CD8a monoclonal antibody (Product # 47-0086-42) in Flow Cytometry" |
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