货号：328107

规格：25 tests

价格：1190

产品类型：流式抗体

品牌：Biolegend

物种：人

宿主：仓鼠

抗体亚型：IgG

荧光染料：FITC

Description

CD90 is a 25-35 kD GPI-anchored protein, also known as Thy-1. It belongs to the Ig superfamily. Human CD90 is expressed on neuronal cells, a subset of CD34+cells, a subset of fetal liver cells and fetal thymocytes, fibroblasts, activated endothelial cells, and some leukemia cell lines. CD34+CD90+cells are primitive hematopoietic stem cells. It has been reported that Thy-1 binds with β2 and β3 integrins and plays bimodal roles in the regulation of cell adhesion and neurite outgrowth, and inhibits hematopoietic stem cells proliferation and differentiation.

• Isotype Control

• FITC Mouse IgG1, κ Isotype Ctrl (FC)

• Reactivity

• Human, African Green, Baboon, Cynomolgus, Pigtailed Macaque, Rhesus, Swine (Pig, Porcine)

• Immunogen

• HEL cells

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).

• Preparation

• The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions. The solution is free of unconjugated FITC.

• Concentration

• Lot-specific (please contacttechnical supportfor concentration and total µg amount, or use ourLookuptool if you have a lot number.)

• Storage & Handling

• The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis.Test size products are transitioning from 20 µl to 5 µl per test. Please check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. Read more atwww.biolegend.com/testsizeregarding the test size change.

• Excitation Laser

• Blue Laser (488 nm)

• Application Notes

• Clone 5E10 recognizes an epitope on Thy-1 independent of its glycosylation, but is abolished under reducing conditions.4Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation1, and immunofluorescence3.

• Application References(PubMed link indicates BioLegend citation)

• 1. Craig W,et al.1993.J. Exp. Med.177:1331. (IP)2. Gundlach CW 4th,et al.2011.Bioconjug. Chem.22:1706. (Pig Reactivity)3. Touboul C,et al.2013.J. Transl. Med.11:28. (IF)4. Bradley JE,et al.2013.Lab Invest.93:365. (Epitope)5. Donnenberg VS,et al.2010.Cytometry B. Clin. Cytom.5:287. (IHC)