| 产品详细信息Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.Applications Reported: This OKT4 (OKT-4) antibody has been reported for use in flow cytometric analysis.Applications Tested: This OKT4 (OKT-4) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 1.Methods in molecular biology (Clifton, N.J.)Ultradense array CGH and discovery of micro-copy number alterations and gene fusions in the cancer genome."45-0048 was used in Immunocytochemistry-immunoflourescence to characterise the protocol steps and analysis required to obtain reliable array comparative array hybridisation results from clinical samples."AuthorsPrzybytkowski E,Aguilar-Mahecha A,Nabavi S,Tonellato PJ,Basik M2.Methods in molecular biology (Clifton, N.J.)Ultradense array CGH and discovery of micro-copy number alterations and gene fusions in the cancer genome."45-0048 was used in Immunocytochemistry-immunoflourescence to characterise the protocol steps and analysis required to obtain reliable array comparative array hybridisation results from clinical samples."AuthorsPrzybytkowski E,Aguilar-Mahecha A,Nabavi S,Tonellato PJ,Basik M3.Frontiers in immunologySchistosoma mansonirSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis."45-0048 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis."AuthorsLopes DM,Oliveira SC,Page B,Carvalho LP,Carvalho EM,Cardoso LS4.BMC biotechnologyEfficient gene transfer into T lymphocytes by fiber-modified human adenovirus 5."45-0048 was used in Flow cytometry/Cell sorting to show the HAdV-11p fiber pseudotyped HAdV-5 could effectively transduce human T cells when human EF1a promoter was used to control the expression of transgene, suggesting its possible application in T cell immunocellular therapy."AuthorsLv Y,Xiao FJ,Wang Y,Zou XH,Wang H,Wang HY,Wang LS,Lu ZZ5.PloS oneCreation of an immunodeficient HLA-transgenic mouse (HUMAMICE) and functional validation of human immunity after transfer of HLA-matched human cells."45-0048 was used in Flow cytometry/Cell sorting to generate a novel immuno-deficient mouse strain expressing human HLA molecules instead of mouse MHC molecules."AuthorsZeng Y,Liu B,Rubio MT,Wang X,Ojcius DM,Tang R,Durrbach A,Ru Z,Zhou Y,Lone YC |
QQ:1749072012