Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-Cyanine5.5

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Mouse IgG2b kappa Isotype Control (eBMG2b), PerCP-Cyanine5.5

货号:45-4732-82

规格:100μg

价格:2365

产品类型:流式抗体

品牌:Thermo Fisher

物种:其它

宿主:小鼠

抗体亚型:IgG2b, kappa

荧光染料:PerCP-Cy5.5

抗体类型 同型对照同型对照:
浓度:0.2 mg/mL用法:Assay-Dependent (Flow);Assay-Dependent (Ctrl)
产品详细信息Description: The monoclonal mouse IgG2b is useful as an isotype control immunoglobulin.Applications Reported: This eBMG2b antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBMG2b antibody has been tested by flow cytometric analysis of normal human peripheral blood and mouse splenocytes. Use at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
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参考文献:
1.Molecular medicine reportsIsolation and characterization of stem cells from differentially degenerated human lumbar zygapophyseal articular cartilage."45-4732 was used in Flow cytometry/Cell sorting to show stem cells with multilineage differentiation potential and clonal properties were identified in human LZAC, and these characteristics were more prominent in mildly degenerated as compared with severely degenerated articular cartilage."AuthorsXiao L,Xu S,Wang X,Jin Z,Wang J,Yang B,Xu H2.Stem cell reportsMIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia."45-4732 was used in Flow cytometry/Cell sorting to identify the hypoxia induced action of MIF as a transcriptional regulator of SOX9 and RUNX2."AuthorsYao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y3.Stem cell research and therapyGlucose regulates tissue-specific chondro-osteogenic differentiation of human cartilage endplate stem cells via O-GlcNAcylation of Sox9 and Runx2."45-4732 was used in Flow cytometry/Cell sorting to show glucose can influence O-GlcNAcylation of Sox9 and Runx2 to regulate differentiation of human cartilage endplate stem cells."AuthorsSun C,Lan W,Li B,Zuo R,Xing H,Liu M,Li J,Yao Y,Wu J,Tang Y,Liu H,Zhou Y4.Stem cell reportsMIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia."45-4732 was used in Flow cytometry/Cell sorting to identify the hypoxia induced action of MIF as a transcriptional regulator of SOX9 and RUNX2."AuthorsYao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y5.PloS oneCreation of an immunodeficient HLA-transgenic mouse (HUMAMICE) and functional validation of human immunity after transfer of HLA-matched human cells."45-4732 was used in Flow cytometry/Cell sorting to generate a novel immuno-deficient mouse strain expressing human HLA molecules instead of mouse MHC molecules."AuthorsZeng Y,Liu B,Rubio MT,Wang X,Ojcius DM,Tang R,Durrbach A,Ru Z,Zhou Y,Lone YC
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