CD3 Monoclonal Antibody (UCHT1), eFluor 506, eBioscience™/CD3 单抗 (UCHT1), eFluor 506, eBioscience™

货号：69-0038-41,69-0038-42

规格：25 tests,100tests

价格：1112,2534

产品类型：流式抗体

品牌：eBioscience

抗原：CD3

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：UCHT1

荧光染料：eFluor 506

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 506, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test（Flow）

产品详细信息Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。

数据

CD3 Antibody (69-0038-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control eFluor® 506 (Product # 69-4714-82) (left) or Anti-Human CD3 eFluor® 506 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.OncoimmunologyAntigen receptor-redirected T cells derived from hematopoietic precursor cells lack expression of the endogenous TCR/CD3 receptor and exhibit specific antitumor capacities."Published figure using CD3 monoclonal antibody (Product # 69-0038-42) in Flow Cytometry"2.The Journal of clinical investigationLoss of ARPC1B impairs cytotoxic T lymphocyte maintenance and cytolytic activity."Published figure using CD3 monoclonal antibody (Product # 69-0038-42) in Flow Cytometry"3.Nature communicationsInflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy."Published figure using CD3 monoclonal antibody (Product # 69-0038-42) in Flow Cytometry"4. PloS oneUlcerative colitis impairs the acylethanolamide-based anti-inflammatory system reversal by 5-aminosalicylic acid and glucocorticoids."Published figure using CD3 monoclonal antibody (Product # 69-0038-42) in Immunofluorescence"5.Hepatology (Baltimore, Md.)Accumulation of natural killer T cells in progressive nonalcoholic fatty liver disease."Published figure using CD3 monoclonal antibody (Product # 69-0038-42) in Immunohistochemistry"

技术参数

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