CD8a Monoclonal Antibody (RPA-T8), Alexa Fluor 532, eBioscience™

货号：58-0088-42

规格：100 Tests

价格：3200

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD8a

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号： RPA-T8

荧光染料：Alexa Fluor 532

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 532, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.03 µg)/test (Flow）

产品详细信息Description:The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RPA-T8 antibody has been pre-titrated and tested y flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 532 nm; Emission: 561 nm; Laser: Green LaserTarget InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD8a Antibody (58-0088-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control Alexa Fluor® 532 (Product # 58-4714-80) (left) or Anti-Human CD8a Alexa Fluor® 532 (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Stem cells internationalUmbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria."Published figure using CD8a monoclonal antibody (Product # 58-0088-42) in Flow Cytometry"2.Central-European journal of immunologyCD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients."Published figure using CD8a monoclonal antibody (Product # 58-0088-42) in Flow Cytometry"3.Molecular medicine reportsCD28 family of receptors on Tcells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade."Published figure using CD8a monoclonal antibody (Product # 58-0088-42) in Flow Cytometry"

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