Pierce NHS-Fluorescein Antibody Labeling Kit
货号：53029
规格：3 reactions
价格：6244
产品类型：蛋白标记
品牌：Thermo Fisher
| The Thermo Scientific NHS-Fluorescein Antibody Labeling Kit is a multi-component kit containing a high-performance derivative of fluorescein dye, activated for easy and reliable labeling of antibodies, proteins and other molecules for use as fluorescent probes.NHS-fluorescein is activated with theN-hydroxy-succinimidyl-ester (NHS ester) functional group. Compared to FITC, the NHS-ester deriviative has greater specificity toward primary amines in the presence of other nucleophiles and results in a more stable linkage following labeling. Pierce Amine-reactive Fluorescein Dyes are mixtures of isomers with reactive groups attached at the 5- and 6-positions of the bottom ring. The properties of these isomers are indistinguishable in terms of excitation and emission spectra, and for protein applications there is no need to isolate a specific isomer. |
| 特点 |
| •Easy—convenient kit with NHS-fluorescein to label and purify antibody in about one hour•Amine-specific labeling—the isothiocyanate variety of fluorescein efficiently labels antibodies and other purified proteins at primary amines (lysine side chains)•Optimized kit procedure—following the standard protocol results in antibodies with excellent dye:protein ratios for optimum activity and fluorescence•Single-use fluors—no need to weigh tiny amounts of powder; kits contain single-use vials of reagent•Efficient purification—kits include purification resin and easy-to-use spin columns, ensuring rapid and efficient removal of non-reacted dye and excellent protein recovery |
| 应用 |
| • Label antibodies for use as immunofluorescent probes• Label oligonucleotides for hybridization probes• Detect proteins in gels and on Western blots |
| 数据 |
Detection of α-tubulin in A549 cells demonstrates use of fluorescein-labeled secondary antibodyCells were grown in 96-well microplates for 18-20 hrs, fixed with 4% paraformaldehyde (Part No. 28906) and permeabilized with 0.1% Surfact-Amps X-100 (Part No. 28314). Cells were then probed with a mouse anti-α-tubulin primary antibody (0.4µg/mL) and Fluorescein-goat anti-mouse secondary antibody (2µg/mL). Nuclei were labeled with Hoechst Dye. Images were acquired by fluorescence microscopy.A.Fluorescence image shows a delicate network of α-tubulin (pseudo-colored green) located exclusively in the cytoplasm.B.Nuclear counterstain with Hoechst Dye (pseudo-colored blue)C.Merged image. Chemical structures of FITC and NHS-FluoresceinBoth of these compounds allow fluorescent labeling of primary amines on proteins and other molecules. |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-26932.htm
QQ:1749072012