CD8a Monoclonal Antibody (SK1), eFluor 450, eBioscience™

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CD8a Monoclonal Antibody (SK1), eFluor 450, eBioscience™

货号:48-0087-42

规格:100tests

价格:2794

产品类型:流式抗体

品牌:Thermo Fisher

抗原:CD8a

物种:人

宿主:小鼠

抗体亚型:IgG1, kappa

克隆号:SK1

荧光染料:eFluor 450

抗体类型:流式抗体同型对照:Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450, eBioscience
浓度: 5 µL/Test用法:5 µL (0.125 µg)/test (Flow)
产品详细信息Description:The SK1 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.Applications Reported: This SK1 antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD8 molecule is composed of two chains termed alpha and beta. CD8 is found on a T cell subset of normal cytotoxic / suppressor cells which make up approximately 20 to 35% of human peripheral blood lymphocytes. The CD8 antigen is also detected on natural killer cells, 80% of thymocytes, on a subpopulation of 30% of peripheral blood null cells and 15 to 30% of bone marrow cells.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
数据

CD8a Antibody (48-0087-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD8a eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis.
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参考文献:
1.The Journal of clinical investigationAlloantigen-specific regulatory T cells generated with a chimeric antigen receptor."48-0087 was used in Flow cytometry/Cell sorting to investigate alloantigen-specific regulatory T cells generated with a chimeric antigen receptor."2.JCI insightSystematic testing and specificity mapping of alloantigen-specific chimeric antigen receptors in regulatory T cells."48-0087 was used in Flow cytometry/Cell sorting to characterise and test alloantigen-specific chimeric antigen receptors in regulatory T cells."3.Scientific reportsCD8+T cells specific for the islet autoantigen IGRP are restricted in their T cell receptor chain usage."48-0087 was used in Flow cytometry/Cell sorting to examine whether there was expansion of clonotypes and sharing of T cell receptor chains in autoreactive CD8+ T cell repertoires."

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