CD7 Monoclonal Antibody (eBio124-1D1 (124-1D1)), eBioscience™

货号：14-0079-82,14-0079-37,14-0079-95

规格：100 µg,2 mg,10 mg

价格：1283,11562,28906

产品类型：流式抗体

品牌：eBioscience

抗原：CD7

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBio124-1D1 (124-1D1)

荧光染料：其它

类型:	一抗	同型对照：	IgG1 kappa
浓度：	0.5 mg/mL	用法：	1.0µg/test（Flow）

产品详细信息Description: The eBio124-1D1 monoclonal antibody reacts with human CD7, also known as gp40 and Leu9. CD7, a 40 kD receptor, is a member of the immunoglobulin gene superfamily. The N-terminal amino acid sequence (aa1-107) is highly homologous to Ig kappa light chain sequence; while the carboxyl-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T lymphocytes,and T cell leukemias. It is also found on natural killer cells, a small suppopulation of normal B cells and on maligant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity, as measured by calcium flux, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation.A clonogenic subpopulation of human CD34(+) CD38(-) cord blood cells that express CD45RA and HLA-DR and high levels of the CD7 has been reported. These cells possess the capacity for lymphopoiesis. They can generate B-cells, natural killer cells, and dendritic cells but do not possess the capacity to develop into myeloid cells or erythroid cells. The CD7(+) phenotype distinguishes primitive human lymphoid progenitors from pluripotent stem cells.Furthermore, it has been suggested that CD7 co-operates with CD28 during Treg function, as mice deficient in both CD28 and CD7 have reduced total numbers of Tregs and these Tregs have reduced suppressive activity.Applications Reported: This eBio124-1D1 (124-1D1) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio124-1D1 (124-1D1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD7 (gp40, Leu9) is a 40 kDa member of the immunoglobulin gene superfamily. CD7 contains N-terminal amino acids 1-107 are highly homologous to Ig kappa-L chains whereas the carboxy-terminal region of the extracellular domain is proline-rich and has been postulated to form a stalk from which the Ig domain projects. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. Further, CD7 is also found on natural killer cells, a small subpopulation of normal B cellsand on malignant B cells. Cross-linking surface CD7 positively modulates T cell and NK cell activity as measured by calcium fluxes, expression of adhesion molecules, cytokine secretion and proliferation. CD7 associates directly with phosphoinositol 3'-kinase. CD7 ligation induces production of D-3 phosphoinositides and tyrosine phosphorylation. Expression of CD7 is an important marker used in leukemia diagnostics.仅用于科研。不用于诊断过程。

数据

CD7 Antibody (14-0079-82) in FlowStaining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 K Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-human CD7 Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4010-82). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Stem cell reportsTargeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis."Published figure using CD7 monoclonal antibody (Product # 14-0079-82) in Flow Cytometry"2.Journal of immunology (Baltimore, Md. : 1950)Adipocyte-derived soluble factor(s) inhibits early stages of B lymphopoiesis."14-0079 was used in Flow cytometry/Cell sorting to investigate the factors contributing to an observed age-related decline in B lymphopoiesis."AuthorsBilwani FA,Knight KL3.Epigenetics and chromatinGeneration of bivalent chromatin domains during cell fate decisions."14-0079 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions."AuthorsDe Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR4.

技术参数

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