CD127 Monoclonal Antibody (eBioRDR5), PE-Cyanine5, eBioscience™

货号：15-1278-42

规格：100 Tests

价格：3050

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD127

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：eBioRDR5

荧光染料：PE-Cy5

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test (Flow）

产品详细信息Description:The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation.Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.Interestingly, recently it has been demonstrated that CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While the co-expression of CD4 and CD25 has become widely used as an indicator of T regs, this method of identification may also include cells without suppressive activity. It has clearly been shown that CD4+CD25+ cells that have down-regulated the expression of CD127 are significantly more highly-enriched for the regulatory T population, as defined by expression of the T reg-specific transcription factor Foxp3 and the suppressive activity of these cells, in vitro.Binding of the eBioRDR5 monoclonal antibody to PBMCs is blocked by pre-incubation of the cells with recombinant human IL-7 (cat. 14-8079).Applications Reported: This eBioRDR5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioRDR5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD127 Antibody (15-1278-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (left) or Anti-Human CD127 PE-Cyanine5 (right).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Journal of immunology (Baltimore, Md. : 1950)Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.2.Frontiers in immunologyATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells."15-1278 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment."3.Cell reportsActivation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells."Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry"4.Acta Cardiologica SinicaThe Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis."Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry"

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