CD4 Monoclonal Antibody (SK3 (SK-3)), Alexa Fluor 532
货号：58-0047-42
规格：100 Tests
价格：3200
产品类型：流式抗体
品牌：Thermo Fisher
物种：人
宿主：小鼠
抗体亚型：IgG1, kappa
荧光染料：Alexa Fluor 532
| 抗体类型 | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor 532 |
| 浓度： | 5 µL/Test | 用法： | 5 μL (0.03 μg)/test (Flow) |
| 产品详细信息Description: The SK3 monoclonal antibody reacts with human CD4, a 59-kDa cell surface receptor expressed by a majority of thymocytes, a subpopulation of mature T helper cells, and at low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). SK3 blocks HIV binding and mixed lymphocyte reaction. The SK3 and RPA-T4 monoclonal antibodies do not cross-block binding, suggesting recognition of distinct epitopes.Applications Reported: This SK3 (SK-3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This SK3 (SK-3) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Alexa Fluor® 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017]. |
| 数据 |
| CD4 Antibody (58-0047-42) in Flow Staining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control Alexa Fluor® 532 (Product # 58-4714-80) (left) or Anti-Human CD4 Alexa Fluor® 532 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
| 1.Clinical cancer research : an official journal of the American Association for Cancer ResearchIpilimumab plus Lenalidomide after Allogeneic and Autologous Stem Cell Transplantation for Patients with Lymphoid Malignancies."58-0047 was used in Flow cytometry/Cell sorting to hypothesize that antitumor-cell responses could be enhanced by the addition of lenalidomide to the cytotoxic T-lymphocyte-associated protein 4 inhibitor ipilimumab."AuthorsKhouri IF,Fernandez Curbelo I,Turturro F,Jabbour EJ,Milton DR,Bassett RL,Vence LM,Allison JP,Gulbis AM,Sharma P2.International journal of immunopathology and pharmacologyCharacteristic patterns of HLA presentation and T cell differentiation in adult-onset Still's disease."Published figure using CD4 monoclonal antibody (Product # 58-0047-42) in Flow Cytometry"3.The Journal of clinical investigationAlloantigen-specific regulatory T cells generated with a chimeric antigen receptor."Published figure using CD4 monoclonal antibody (Product # 58-0047-42) in Flow Cytometry"4.Scientific reportsCIB1 and CIB2 are HIV-1 helper factors involved in viral entry."Published figure using CD4 monoclonal antibody (Product # 58-0047-42) in Flow Cytometry"5.The Journal of clinical investigationAlloantigen-specific regulatory T cells generated with a chimeric antigen receptor."Published figure using CD4 monoclonal antibody (Product # 58-0047-42) in Flow Cytometry" |
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