CD28 Monoclonal Antibody (CD28.2), APC

货号：17-0289-41,17-0289-42

规格：25 T,100 T

价格：1215,2629

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD28

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：CD28.2

荧光染料：APC

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test(Flow)

产品详细信息Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The CD28.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

数据

CD28 Antibody (17-0289-41) in FlowNormal human peripheral blood cells were stained with CD19 Monoclonal Antibody, FITC (Product # 11-0199-42) and Mouse IgG1 kappa Isotype Control, APC (Product # 17-4714-82) (left) or CD28 Monoclonal Antibody, APC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Age (Dordrecht, Netherlands)Transcriptomic and epigenetic analyses reveal a gender difference in aging-associated inflammation: the Vitality 90+ study.2. Journal of immunology (Baltimore, Md. : 1950)Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.3. Age (Dordrecht, Netherlands)The trajectory of the blood DNA methylome ageing rate is largely set before adulthood: evidence from two longitudinal studies.4. Age (Dordrecht, Netherlands)Transcriptomic and epigenetic analyses reveal a gender difference in aging-associated inflammation: the Vitality 90+ study.5. BMC genomicsAgeing-associated changes in the human DNA methylome: genomic locations and effects on gene expression.

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