FOXP3 Monoclonal Antibody (236A/E7), eFluor 660, eBioscience™

货号：50-4777-41

规格：100 Tests

价格：0

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：236A/E7

荧光染料：eFluor 660

类型:	一抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 660, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test（Flow）

产品详细信息Description: The 236A/E7 antibody reacts with human Foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of Foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining and flow cytometric analysis of freshly isolated human peripheral blood mononuclear cells (PBMCs) with the 236A/E7 antibody using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol reveals staining of the CD4+CD25bright population.The epitope from 236A/E7 is different from that of PCH101 (cat. 72-5776). This antibody has also been shown to recognize rhesus macaque, sooty mangabey and cynomolgus macaque.Applications Reported: This 236A/E7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This 236A/E7 antibody has been pre-titrated and tested using the Foxp3/Transctiption Factor Staining Buffer Set (cat. 00-5523) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (50-4777-42) in FlowIntracellular staining of normal human peripheral blood cells with Anti-Human/Non-Human Primate CD25 PE (Product # 12-0257-42) and Mouse IgG1 K Isotype Control eFluor® 660 (Product # 50-4714-82) (left) or Anti-Human Foxp3 eFluor® 660 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.FOXP3 Antibody (50-4777-42)Frontiers in oncology 2020 -Figure 1 Multiplex IHC on tissue before and after sepsis. (A) Images showing T cell infiltration and macrophage infiltration in tissue before (omentum biopsy) and after (tumor resection) sepsis. CKAE1/AE3 was used to visualize tumor cells. Effector T cells were characterized as CD3 + CD8 + , regulatory T cells as CD3 + Foxp3 + , M1 macrophages as CD68 + , and M2 macrophages as CD163 + . (B) CD8 T cells, T Regs , M1 and M2 macrophages per mm 2 . Immune cells were quantified in control tissue, and in tissue obtained before and after sepsis. (C) CD8 effector T cell/T Reg ratio and M1/M2 ratio before and after sepsis. (D) Average staining intensity of immune cell markers before and after sepsis. Unstained tissue was used as control. Untreated primary tumor from stage III and IV ovarian cancer patient were used as control ( n = 16). Red line indicates mean.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献

1.Experimental hematology and oncologyChanges in peripheral blood lymphocytes in polycythemia vera and essential thrombocythemia patients treated with pegylated-interferon alpha and correlation withJAK2V617Fallelic burden."Published figure using FOXP3 monoclonal antibody (Product # 50-4777-42) in Flow Cytometry"2.Nature medicineA human anti-IL-2 antibody that potentiates regulatory T cells by a structure-based mechanism."50-4777 was used in Flow cytometry/Cell sorting to suggest that IL-2-F5111.2 may provide an immunotherapy to treat autoimmune diseases and graft-versus-host disease."AuthorsTrotta E,Bessette PH,Silveria SL,Ely LK,Jude KM,Le DT,Holst CR,Coyle A,Potempa M,Lanier LL,Garcia KC,Crellin NK,Rondon IJ,Bluestone JA3.OncotargetHDAC inhibition potentiates immunotherapy in triple negative breast cancer."Published figure using FOXP3 monoclonal antibody (Product # 50-4777-42) in Flow Cytometry"4.Cell researchBlocking the recruitment of naive CD4+T cells reverses immunosuppression in breast cancer."Published figure using FOXP3 monoclonal antibody (Product # 50-4777-42) in Flow Cytometry"5.International journal of molecular medicineAltered expression of miR-92a correlates with Th17 cell frequency in patients with primary biliary cirrhosis."Published figure using FOXP3 monoclonal antibody (Product # 50-4777-42) in Flow Cytometry"

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