CD28 Monoclonal Antibody (CD28.2), APC-eFluor 780

货号：47-0289-42

规格：100 T

价格：3200

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：CD28.2

荧光染料：其它

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor 780
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test(Flow)

产品详细信息Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: This CD28.2 antibody has been reported for use in flow cytometric analysis.Applications Tested:This CD28.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

数据

CD28 Antibody (47-0289-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 eFluor® 450 (Product # 48-0199-42) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD28 APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis. CD28 Antibody (47-0289-42)Molecular medicine reports 2016 -Published figure using CD28 monoclonal antibody (Product # 47-0289-42)

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参考文献

1. Journal of immunology (Baltimore, Md. : 1950)Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.2. Journal for immunotherapy of cancerDetailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.3. AutophagyA novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.4. AutophagyA novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.5. Journal of immunology (Baltimore, Md. : 1950)Aging and cytomegalovirus infection differentially and jointly affect distinct circulating T cell subsets in humans.

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