CD28 Monoclonal Antibody (CD28.2), eFluor 450

货号：48-0289-42

规格：100 T

价格：2895

产品类型：流式抗体

品牌：eBioscience

抗原：CD28

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：CD28.2

荧光染料：eFluor 450

类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 450
浓度：	5 µL/Test	用法：	5 µL (0.125 µg)/test(Flow)

产品详细信息Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: This CD28.2 antibody has been reported for use in flow cytometric analysis.Applications Tested:This CD28.2 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing Tcells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.

数据

CD28 Antibody (48-0289-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD19 APC (Product # 17-0199-42) and Mouse IgG1 K Isotype Control eFluor® 450 (Product # 48-4714-82) (left) or Anti-Human CD28 eFluor® 450 (right). Cells in the lymphocyte gate were used for analysis. CD28 Antibody (48-0289-42)Molecular medicine reports 2016 -Published figure using CD28 monoclonal antibody (Product # 48-0289-42)

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参考文献

1. AutophagyA novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.2. Molecular medicine reportsCD28 family of receptors on Tcells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.3. Journal for immunotherapy of cancerDetailed characterization of tumor infiltrating lymphocytes in two distinct human solid malignancies show phenotypic similarities.4. Cell reportsHuman Liver Memory CD8+T Cells Use Autophagy for Tissue Residence.5. The Journal of biological chemistryN-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24.

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