FOXP3 Monoclonal Antibody (PCH101), Alexa Fluor 488, eBioscience™

货号：53-4776-42,53-4776-41

规格：100Tests,25Tests

价格：3481,1752

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：PCH101

荧光染料：Alexa Fluor 488

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor 488, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test（Flow）

产品详细信息Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is amember of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Buffer Staining Set (cat. 00-5523) and Protocol. Please refer to Best Protocol of normal human peripheral blood cells using the Foxp3 Buffers and protocol. Please refer to Best Protocols for instructions. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (53-4776-42) in FlowNormal human peripheral blood cells were surface-stained with Anti-Human CD4 PE (Product # 12-0049-42) (left) and Anti-Human CD25 APC (Product # 17-0259-42) (right) followed by fixation and permeabilization using Foxp3/Transcription Factor Buffer Staining Set (Product # 00-5523-00) and subsequently stained with Anti-Human Foxp3 Alexa Fluor® 488. Cells in the lymphocyte gate were used for analysis. FOXP3 Antibody (53-4776-42)Frontiers in immunology 2020 -Figure 4 Follicle-like structures of SPMS brains exhibit CD3 + CD4 + T cells, which neither express PD-1 nor FOXP3. (A-E) IF staining of CD3, CD4 and PD-1 reveal CD3 + CD4 + PD-1 - T-helper cells in progressive MS. Inserts in the upper right corners show magnification of the white box. (F-I) IF staining of CD3 and FOXP3 on serial sections of a representative meningeal follicle-like structure in SPMS (same region as Figure 3 ). CD3 + T cells, but no FOXP3 + cells were detected. Inserts show magnification of the white box. Scale bars indicate 100 mum, inserts 10 mum.

推荐产品：

-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献

1.Investigative ophthalmology and visual scienceT-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma."Published figure using FOXP3 monoclonal antibody (Product # 53-4776-42) in Flow Cytometry"2.Journal of veterinary internal medicineCan levamisole upregulate the equine cell-mediated macrophage (M1) dendritic cell (DC1) T-helper 1 (CD4 Th1) T-cytotoxic (CD8) immune response in vitro?"53-4776 was used in Flow cytometry/Cell sorting to study the effects and mechanism of action of levamisole in horses."AuthorsWitonsky S,Buechner-Maxwell V,Santonastasto A,Pleasant R,Werre S,Wagner B,Ellison S,Lindsay D3.Experimental and therapeutic medicineEffect of TGF-β1 on blood CD4+CD25highregulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis."Published figure using FOXP3 monoclonal antibody (Product # 53-4776-42) in Flow Cytometry"4.Journal of immunology (Baltimore, Md. : 1950)Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.AuthorsLim HW,Hillsamer P,Banham AH,Kim CH5.BloodIL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients."Published figure using FOXP3 monoclonal antibody (Product # 53-4776-42) in Flow Cytometry"

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