FOXP3 Monoclonal Antibody (PCH101), PE-Cyanine5, eBioscience™

货号：15-4776-42,15-4776-41

规格：100 Tests,25Tests

价格：4005,2200

产品类型：流式抗体

品牌：eBioscience

抗原：FOXP3

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：PCH101

荧光染料：PE-Cy5

类型:	一抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5, eBioscience™
浓度：	5 µL/Test	用法：	5 µL (0.25 µg)/test

产品详细信息Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is amember of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, cat. 11-0048, or RPA-T4, cat. 11-0049, depending on the species) and CD25 (BC96, cat. 17-0259).Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cellsusing the Foxp3/Transcription Factor Buffer Set and protocol. Please refer to Best Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.仅用于科研。不用于诊断过程。

数据

FOXP3 Antibody (15-4776-42) in FlowStaining of normal human peripheral blood mononuclear cells with Anti-Human CD4 FITC (Product # 11-0049-42) and Rat IgG2a K Isotype Control PE-Cyanine5 (Product # 15-4321-80) (left) or Anti-Human Foxp3 PE-Cyanine5 (right) using the Foxp3/Transcription Factor Buffer Set.Cells in the lymphocyte gate were used for analysis.

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-Intracellular Fixation & Permeabilization Buffer Set（货号#88-8824）-Foxp3 / Transcription Factor Staining Buffer Set（货号#00-5523-00）-Flow Cytometry Staining Buffer（货号#00-4222-57/26）

参考文献

1.BloodMucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.AuthorsEpple HJ,Loddenkemper C,Kunkel D,Tröger H,Maul J,Moos V,Berg E,Ullrich R,Schulzke JD,Stein H,Duchmann R,Zeitz M,Schneider T2.Investigative ophthalmology and visual scienceT-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma."Published figure using FOXP3 monoclonal antibody (Product # 15-4776-42) in Flow Cytometry"3.Experimental and therapeutic medicineEffect of TGF-β1 on blood CD4+CD25highregulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis."Published figure using FOXP3 monoclonal antibody (Product # 15-4776-42) in Flow Cytometry"4.Cancer cellFc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies."Published figure using FOXP3 monoclonal antibody (Product # 15-4776-42) in Flow Cytometry"5.BloodIL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.

技术参数

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