CD69 Monoclonal Antibody (FN50), APC-eFluor 780, eBioscience™

货号：47-0699-42

规格：100tests

价格：2754

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD69

物种：人

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：FN50

荧光染料：APC-eFluor 780

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor 780, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test (Flow）

产品详细信息Description:The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.Applications Reported: The FN50 antibody has been reported for use in flow cytometric analysis.Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red LaserTarget InformationCD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD69 Antibody (47-0699-42) in FlowStaining of unstimulated (left) or overnight PHA-stimulated (Product # 00-4977-93) normal human peripheral blood cells with CD3 Monoclonal Antibody, PE (Product # 12-0037-42) and Mouse IgG1 K Isotype Control, APC-eFluor® 780 (Product # 47-4714-82) (left) or CD69 Monoclonal Antibody, APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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