CD69 Monoclonal Antibody (FN50), PE, eBioscience™

货号：12-0699-41,12-0699-42

规格：25tests,100tests

价格：909,2230

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD69

物种：人/恒河猴

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：FN50

荧光染料：PE

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.015 µg)/test (Flow）

产品详细信息Description:The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.Applications Reported: The FN50 antibody has been reported for use in flow cytometric analysis.Applications Tested: This FN50 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.015 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD69 (AIM, Active Inducer Molecule) is a gp28/34 disulfide bonded homodimer with a molecular weight of 60 kDa under non-reducing conditions. CD69 contains one or two N linked oligosacaride and the molecule is present on activated platelets. In normal peripheral blood a variable percentage of cells express the CD69 antigen, and it is involved in lymphocyte signal transduction. Expression CD69 is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. Alternative splicing results in multiple transcript variants of CD69. Diseases associated with CD69 dysfunction include coccidiodomycosis and asthma.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD69 Antibody (12-0699-41) in FlowStaining of overnight PHA-stimulated normal human peripheral blood cells with Anti-Human CD3 eFluor® 450 (Product # 48-0037-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD69 PE (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.PloS oneHeterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization."12-0699 was used in Flow cytometry/Cell sorting to investigate IL-17A responses against Salmonella enterica serovar Typhi antigens in T memory subsets."2.eLifeHIV Tat controls RNA Polymerase II and the epigenetic landscape to transcriptionally reprogram target immune cells."12-0699 was used in Flow cytometry/Cell sorting to investigate target genes and molecular mechanisms by which Tat modulates transcription."3.Science signalingThe 4E-BP-eIF4E axis promotes rapamycin-sensitive growth and proliferation in lymphocytes."12-0699 was used in Flow cytometry/Cell sorting to investigate the mechanism by which rapamycin leads to selective immunosupression of lymphocytes, showing that the 4E-BP-efF4E axis promotes growth and proliferation of lymphocytes and is uniquely sensitive to rapamycin in these cells."

技术参数

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