CD152 (CTLA-4) Monoclonal Antibody (14D3), PerCP-eFluor 710, eBioscience™

货号：46-1529-41

规格：25tests

价格：1267

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD152

物种：其它

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：14D3

荧光染料：Alexa Fluor 488

抗体类型:	流式抗体	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PerCP-eFluor 710, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.03 µg)/test (Flow）

产品详细信息Description:The 14D3 monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cellsto an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.The 14D3 antibody also recognizes rhesus monkey and has inhibitor activity.Applications Reported: This 14D3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 14D3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD152 (CTLA-4) Antibody (46-1529-41) in FlowPHA-stimulated human peripheral blood cells were intracellularly stained with Mouse IgG2a K Isotype Control PerCP-eFluor® 710 (Product # 46-4724-82) (blue histogram) or Anti-Human CD152 (CTLA-4) PerCP-eFluor® 710 (purple histogram) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.Nature communicationsFollicular regulatory T cells impair follicular T helper cells in HIV and SIV infection."46-1529 was used in Flow cytometry/Cell sorting to determine TFR contribute to inefficient germinal centre responses and inhibit HIV and SIV clearance."2.OncotargetHDAC inhibition potentiates immunotherapy in triple negative breast cancer."Published figure using CD152 (CTLA-4) monoclonal antibody (Product # 46-1529-42) in Flow Cytometry"3.OncotargetPreferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.

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