CD154 (CD40 Ligand) Monoclonal Antibody (24-31), FITC, eBioscience™
货号：11-1548-42
规格：100tests
价格：2580
产品类型：流式抗体
品牌：Thermo Fisher
抗原：CD154
物种：其它
宿主：小鼠
抗体亚型：IgG1, kappa
克隆号：24-31
荧光染料：FITC
| 抗体类型: | 流式抗体 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience |
| 浓度： | 5 µL/Test | 用法： | 5 µL (1 µg)/test (Flow） |
产品详细信息Description:The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activationand induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.Applications Reported: 24-31 has been reported for use in flow cytometric analysis.Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据 |
| CD154 (CD40 Ligand) Antibody (11-1548-42) in FlowHuman peripheral blood cells unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (500X) (Product # 00-4970-03) (right) were stained with Anti-Human CD8a PE (Product # 12-0088-80) and Anti-Human CD154 (CD40 Ligand) FITC. Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Scientific reportsIntracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding."11-1548 was used in Flow cytometry/Cell sorting to show microfluidic vortex shedding mediates intracellular delivery of mRNA into primary human T lymphocytes."2.Oncology lettersPhotodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70."Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 11-1548-42) in Flow Cytometry" |
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