CD154 (CD40 Ligand) Monoclonal Antibody (24-31), PE-eFluor 610, eBioscience™

货号：61-1548-42

规格：100 Tests

价格：3090

产品类型：流式抗体

品牌：Thermo Fisher

抗原：CD154

物种：人/小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：24-31

荧光染料：PE-eFluor 610

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor 610, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.5 µg)/test (Flow）

产品详细信息Description:The 24-31 monoclonal antibody reacts with human CD154, a 39 kDa transmembrane glycoprotein also known as gp39 and CD40 ligand (CD40L). CD154 is a member of the TNF superfamily and is expressed transiently by activated T cells. Through its binding to CD40 on antigen presenting cells including B cells, monocytes/macrophages and dendritic cells, CD154 serves a crucial function in T-APC cognate interaction. CD154 interaction with CD40 transduces signals for T-dependent B-cell activationand induces B cell cycle entry. 24-31 cross-reacts with the cynomolgus monkey CD154.Applications Reported: This 24-31 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 24-31 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD154 (CD40 Ligand) Antibody (61-1548-42) in FlowNormal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (500X) (Product # 00-4970-03) (right) and stained with Anti-Human CD8a eFluor® 450 (Product # 48-0088-42) and Anti-Human CD154 (CD40 Ligand) PE-eFluor® 610. Total viable cells were used for analysis.

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参考文献：

1.Oncology lettersPhotodynamic therapy reduces the inhibitory effect of osteosarcoma cells on dendritic cells by upregulating HSP70."Published figure using CD154 (CD40 Ligand) monoclonal antibody (Product # 61-1548-42) in Flow Cytometry"

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