EZ-Link Hydrazide-Biocytin
货号：28020
规格：25 mg
价格：0
产品类型：蛋白标记
品牌：Thermo Fisher
| Thermo Scientific EZ-Link Hydrazide-Biocytin is a mid-length, hydrazide-activated biotinylation reagent that contains an extra amine for greater reagent solubility during labeling reactions with carbonyl-containing compounds.Hydrazide-activated biocytin enables simple and efficient biotin labeling of polyclonal antibodies and other glycoproteins. Mild oxidation of sialic acid sugars with sodium periodate produces reactive aldehydes that can be conjugated to hydrazides. This compound is identical toBiotin-LC-Hydrazide (Part No. 21340), except that it possesses an additional primary amine in its spacer arm. This makes the reagent slightly more soluble and also makes possible other labeling and conjugation applications.We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.Biotinylation reagentsdiffer in reactivity, length, solubility, cell permeability and cleavability. Hydrazides and alkoxyamines are twotypes of carbonyl-reactive groups. Hydrazides (—NH-NH2) react specifically with aldehyde groups in slightly acidic conditions to form hydrazone linkages; these can be further reduced to stable secondary amine bonds usingsodium cyanoborohydride (Part No. 44892). The reaction is more efficient in the presence ofaniline (Part No. 88944). Alternatively, hydrazides can be conjugated to carboxylic acids usingEDC carbodiimide chemistry.Reactive aldehyde groups can be generated in glycoproteins and other polysaccharide compounds by oxidation of constituent sugar diols usingsodium periodiate (Part No. 20504). Sialic acid residues are common components of protein glycosylation and are easily converted to aldehydes with 1 mM NaIO4. |
| 特点 |
| •Glycoprotein labeling—biotinylate glycosylated proteins at sialic acid residues for detection or purification using streptavidin probes or resins•Cell surface labeling—biotinylate and isolate cell surface glycoproteins; reagent does not permeate membranes of whole cells•Aldehyde-reactive—reacts with aldehydes formed by periodate-oxidation of sugar groups•Hydrazide-activated—perform reactions at pH 4 to 6 in buffers such as sodium acetate•Irreversible—forms semi-permanent hydrazone bonds; spacer arm cannot be cleaved•Solubility—usually dissolved in DMSO before further dilution in aqueous buffers•Medium length—spacer arm (total length added to target) is 19.7 angstroms; contains an additional primary amine, which can be targeted as a second conjugation point |
| 应用 |
| • Labeling erythrocyte membranes• Labeling cytosine residues in DNA and RNA via a bisulfite-catalyzed transamination reaction• Labeling of resolved glycoproteins on blots using either a galactose oxidase or a periodate oxidation method |
| 数据 |
Chemical structure of Hydrazide-Biocytin |
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