CD45RA Monoclonal Antibody (HI100), PE-eFluor 610, eBioscience™
货号:61-0458-42,61-0458-41
规格:100Tests,25Tests
价格:3107,1244
产品类型:流式抗体
品牌:eBioscience
抗原: CD45RA
物种:人
宿主:小鼠
抗体亚型:IgG2b, kappa
克隆号:HI100
荧光染料:PE-eFluor 610
抗体类型: | 单抗 | 同型对照: | Mouse IgG2b kappa Isotype Control (eBMG2b), PE-eFluor 610, eBioscience™ |
浓度: | 5uL/test | 用法: | 5uL(0.03µg)/test(Flow) |
产品详细信息Description: The HI100 monoclonal antibody reacts with human CD45RA, a 220 kDa molecule expressed by subpopulations of CD4+ peripheral T lymphocytes, CD8+ peripheral T lymphocytes, and B cells. The CD45RA+ T cell populations are mainly naive/virgin allowing the use of HI100 mAb as a phenotypic marker to discriminate T cell subsets.Applications Reported: This HI100 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI100 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.仅用于科研。不用于诊断过程。
数据 |
CD45RA Antibody (61-0458-42) in FlowStaining of normal human peripheral blood cells with Anti-Human CD45RO APC (Product # 17-0457-42) and Mouse IgG2b K Isotype Control PE-eFluor® 610 (Product # 61-4732-82) (left) or Anti-Human CD45RA PE-eFluor® 610 (right). Cells in the lymphocyte gate were used for analysis.CD45RA Antibody (61-0458-42)Molecular medicine reports 2019 -Figure 1. Purity of CD3 + CD4 + CD45RA + T cells, CD3 + CD8 + T cells and CD4 + CD25 + FoxP3 + Treg cells. CD3 + CD4 + CD45RA + T cells and CD3 + CD8 + T cells were purified by magnetic cell sorting, and their purity was determined by flow cytometry. (A) The purity of CD3 + CD4 + CD45RA + T cells was 99.45% (CD3 + T cells, 99.6%; CD4 + CD45RA + T cells, 99.85%). (B) The purity of CD3 + CD8 + T cells was 95.32%. (C) The purity of CD4 + CD25 + FoxP3 + Treg cells was 93.15% (CD4 + T cells, 99.5%; CD25 + FoxP3 + T cells, 93.62%). (D) miR-155 expression in T cells of the patients with vitiligo and healthy donor was detected by reverse transcription quantitative polymerase chain reaction. **P<0.01 vs. control (healthy donor). Treg, regulatory T; miR-155, microRNA-155; FoxP3, forkhead box P3. |
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参考文献: |
1.NatureTargeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection."Published figure using CD45RA monoclonal antibody (Product # 61-0458-42) in Flow Cytometry"2.Immunologic researchThe distribution and function of human memory T cell subsets in lung cancer."Published figure using CD45RA monoclonal antibody (Product # 61-0458-42) in Flow Cytometry"3.Immunology and cell biologyModeling the dynamics of neonatal CD8+T-cell responses."61-0458 was used in Flow cytometry/Cell sorting to analyse the dynamics of neonatal and adult CD8 T cell responses following in vitro stimulation and in vivo infection."AuthorsReynaldi A,Smith NL,Schlub TE,Venturi V,Rudd BD,Davenport MP4.Immunology and cell biologyModeling the dynamics of neonatal CD8+T-cell responses."61-0458 was used in Flow cytometry/Cell sorting to analyse the dynamics of neonatal and adult CD8 T cell responses following in vitro stimulation and in vivo infection."AuthorsReynaldi A,Smith NL,Schlub TE,Venturi V,Rudd BD,Davenport MP5.NatureTargeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection."Published figure using CD45RA monoclonal antibody (Product # 61-0458-42) in Flow Cytometry" |
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