Rabbit anti Human interleukin-17F antibody
货号：AHP1283
规格：0.1 mg
价格：4063
产品类型：免疫组化一抗
品牌：Bio-Rad
抗原：interleukin-17F
物种：人
宿主：兔
抗体亚型：IgG
荧光染料：Purified
| 产品介绍 | | |
| Rabbit anti Human interleukin-17F antibodyrecognizes human IL-17F (interleukin-17F), a disulphide-linked homodimer consisting of two 133 amino acid chains, which belongs to the IL-17 gene family and shares the highest sequence homology with IL-17A.IL-17F is expressed by activated CD4+ T-cells and monocytes, and acts as a stimulator of cytokine production, including granulocyte colony-stimulating factor (GM-CSF), IL-6 and IL-8. IL-7F also stimulates the production of IL-2, MCP-1 (monocyte chemotactic protein-1) and TGFB (Transforming growth factor beta) by endothelial cells, and inhibits endothelial cell angiogenesis.Rabbit anti Human interleukin-17F antibody (AHP1283) has been used in neutralisation assays where 50% inhibition (ND50) of the biological activity of 50ng/ml of human IL-17F was achieved using 0.9-1.2μg/ml of antibody. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified IgG - lyophilized |
| Reconstitution | Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add sodium azide. |
| Antiserum Preparation | Antisera to human IL-17F were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography. |
| Buffer Solution | Phosphate buffered saline |
| Preservative Stabilisers | None present |
| Immunogen | Recombinant human IL-17F. |
| Approx. Protein Concentrations | IgG concentration 1.0mg/ml |
| Regulatory | For research purposes only |
| Guarantee | 12 months from date of despatch |
| 存储条件 | | |
| Prior to reconstitution store at -20°C.After reconstitution store at -20°C.This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| ELISA | √ | 0.5 | 2.0ug/ml |
| Functional Assays | √ | 0.9 | 1.2ug/ml |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. |
| 二抗 |
| Description | Product Code | Applications | Pack Size |
| Goat anti Rabbit IgG (Fc):Biotin | STAR121B | E,WB | 1mg |
| Goat anti Rabbit IgG (H/L):HRP | STAR124P | C,E,WB | 1mg |
| 文献 |
| 1.Starnes, T.et al.(2001) Cutting edge: IL-17F, a novel cytokine selectively expressed in activated T cells and monocytes, regulates angiogenesis and endothelial cell cytokine production.J Immunol. 167 (8): 4137-40. |
技术参数 产品应用 elisa；wb
产品类型 Polyclonal Antibody
推荐用于 elisa；wb
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