CD3e Monoclonal Antibody (eBio500A2 (500A2)), Super Bright 436, eBioscience™

货号：62-0033-82

规格：100 µg

价格：2763

产品类型：流式抗体

品牌：eBioscience

抗原：CD3e

物种：小鼠

宿主：其它

抗体亚型：IgG

克隆号：eBio500A2 (500A2)

荧光染料：Super Bright 436

类型:	一抗	同型对照：	IgG
浓度：	0.2 mg/mL	用法：	1 µg/test（Flow）

产品详细信息Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Reported: This eBio500A2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio500A2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。

数据

CD3e Antibody (62-0033-82) in FlowBALB/c mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, FITC (Product # 11-0452-82) (left) and 0.5 µg of CD3e Monoclonal Antibody, Super Bright 436 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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技术参数

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