Rat IgG2a kappa Isotype Control (eBR2a), FITC

货号：11-4321-41,11-4321-42

规格：25 T,100 T

价格：851,1492

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：eBR2a

荧光染料：FITC

类型:	同型对照	同型对照：
浓度：	5 µL/Test	用法：	Assay-Dependent(Flow)；Assay-Dependent(IHC)；Assay-Dependent(Ctrl)；Assay-Dependent(ICC)；Assay-Dependent(IF)

产品详细信息Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: The FITC conjugated rat IgG2a isotype control is offered in 2 formats:- µg size: This rat IgG2a isotype control has been tested by flow cytometric analysis of mouse splenocytes. This can be used at the sameconcentration as the experimental antibody.- test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells and can be used at 5 µL for the catalog number ending in -41 or -42 and 20µL/test for the product ending in -73 per test. Each test size contains 0.5 µg.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Rat IgG2a kappa Isotype Control (11-4321-42) in FlowC57Bl/6 splenocytes stimulated for 2 days with plate-bound Anti-Mouse CD3e Functional Grade Purified (Product # 16-0031-82) were surface stained with Anti-Mouse CD19 PE (Product # 12-0193-82). Cells were then fixed and permeabilized using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and stained intracellularly with 0.125 µg of Rat IgG2a K Isotype Control FITC (Product # 11-4321-42) (left) or 0.125 µg of Anti-Mouse/Rat Ki-67 FITC (right).

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技术参数

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