Rabbit IgG Isotype Control, FITC

admin 2024-10-18 11

Rabbit IgG Isotype Control, FITC

货号:11-4614-80

规格:25 µg

价格:822

产品类型:同型对照

品牌:eBioscience

物种:其它

宿主:兔

抗体亚型:IgG

荧光染料:FITC

抗体类型 同型对照同型对照:
浓度:0.5 mg/mL用法:Assay-Dependent (Flow);Assay-Dependent (Ctrl)
产品详细信息Description: The rabbit IgG polyclonal antibody is useful as an isotype control immunoglobulin.Applications Reported: The Rabbit IgG isotype control has been reported for use as a negative control for immunofluorescent staining and flow cytometric analysis.Applications Tested: This isotype control antibody has been tested by flow cytometric analysis. Please use at the same concentration as the experimental antibody.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.
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参考文献:
1.Tissue engineering. Part AEpithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds."11-4614 was used in Flow cytometry/Cell sorting to show the capacity for human mesenchymal stromal cells to differentiate toward lung epithelial phenotypes."2.BMC research notesA method for quantifying pulmonary Legionella pneumophila infection in mouse lungs by flow cytometry."11-4614 was used in Flow cytometry/Cell sorting to describe a novel, rapid and versatile method that detects bacteria phagocytosed by neutrophils."3.The Journal of clinical investigationSchlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia."11-4614 was used in Flow cytometry/Cell sorting to study the involvement of SLFN4+ myeloid-derived suppressor cells in gastric tumorigenesis following chronic Helicobacter pylori infection."

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