Mouse IgM Isotype Control (11E10), PE-Cyanine7
货号：25-4752-81
规格：50 μg
价格：1126
产品类型：小鼠同型对照
品牌：eBioscience
物种：其它
宿主：小鼠
抗体亚型：IgM
荧光染料：PE-Cy7
| 抗体类型 | 同型对照 | 同型对照： | |
| 浓度： | 0.2 mg/mL | 用法： | Assay-Dependent (Flow)；Assay-Dependent (Ctrl) |
| 产品详细信息Description: The mouse IgM is useful as an isotype control immunoglobulin. The immunoglobulin was generated by immunization with lipopolysaccharide (LPS). The immunoglobulin is reactive with LPS.Applications Reported: Mouse IgM Isotype Control PE-Cyanine7 has been reported for use in flow cytometric analysis.Applications Tested: Mouse IgM Isotype Control PE-Cyanine7 has been tested by flow cytometric analysis of mouse splenocytes and normal human peripheral blood cells. Use at the same concentration as the experimental antibody.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser靶标信息IgM (Immunoglobulin M) is expressed intracellularly during the early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. The isotype of a primary antibody and its application can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are also useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody. |
| 数据 |
| Mouse IgM Isotype Control (25-4752-81) in FlowHuman peripheral blood cells were unstained (left) or stained with 0.125 µg of Mouse IgM Isotype Control, PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis. |
| 推荐产品： |
| 二抗荧光二抗免疫组化一抗标签抗体 |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-27427.htm
QQ:1749072012