CD4 Monoclonal Antibody (GK1.5), Biotin, Functional Grade,

货号：36-0041-85

规格：500 µg

价格：3669

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：GK1.5

荧光染料：Alexa Fluor 488

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), Biotin
浓度：	0.5 mg/mL	用法：	0.125 µg/test(Flow)；Assay-Dependent(IHC (F))；Assay-Dependent(IP)；Assay-Dependent(FN)；Assay-Dependent(Ctrl)

产品详细信息Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in isolation and depletion of CD4+ cells, and for blocking of CD4-ligand binding and T cell activation that occurs through CD4.Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.05 ng/ug cytokine as determined by the LAL assay.Storage and handling: Use in a sterile environment.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

数据

CD4 Antibody (36-0041-85) in FlowStaining of C57BL/6 splenocytes with 0.125 µg of Rat IgG2b kappa Isotype Control Functional Grade Biotin (Product # 16-4031-81) (open histogram) or 0.125 µg of Anti-Mouse CD4 Functional Grade Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. CD4 Antibody (36-0041-85)Staining of mouse splenocytes. As expected based on known relative expression patterns, CD4 clone GK1.5 stains about 25% of total lymphocytes and does not stain CD45R (B220)+ B cells. Details: Balb/c splenocytes were surface stained with CD45R (B220, clone RA3-6B2) and Rat IgG2b Isotype Control or CD4 (clone GK1.5). Cells in the lymphocyte gate were used for analysis.

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参考文献

1. Journal of immunology (Baltimore, Md. : 1950)Multispectral Imaging of T and B Cells in Murine Spleen and Tumor.2. Scientific reportsHeavy Chain-Hyaluronan/Pentraxin 3 from Amniotic Membrane Suppresses Inflammation and Scarring in Murine Lacrimal Gland and Conjunctiva of Chronic Graft-versus-Host Disease.3. Gene therapyEffects of irradiating adult mdx mice before full-length dystrophin cDNA transfer on host anti-dystrophin immunity.4. Frontiers in physiologyShifts in the Gut Microbiota Composition Due to Depleted Bone Marrow Beta Adrenergic Signaling Are Associated with Suppressed Inflammatory Transcriptional Networks in the Mouse Colon.5. Scientific reportsHeavy Chain-Hyaluronan/Pentraxin 3 from Amniotic Membrane Suppresses Inflammation and Scarring in Murine Lacrimal Gland and Conjunctiva of Chronic Graft-versus-Host Disease.

技术参数

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