CD4 Monoclonal Antibody (RM4-4), PE-eFluor 610
货号：61-0043-80,61-0043-82
规格：25 ug,100 μg
价格：1294,2919
产品类型：流式抗体
品牌：eBioscience
抗原：CD4
物种：小鼠
宿主：大鼠
抗体亚型：IgG2a, kappa
克隆号：RM4-4
荧光染料：PE-eFluor 610
| 类型: | 单抗 | 同型对照： | Rat IgG2b kappa Isotype Control (eB149/10H5), PE-eFluor 610 |
| 浓度： | 0.2 mg/mL | 用法： | 0.25 µg/test(Flow) |
产品详细信息Description: The RM4-4 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by the majority of thymocytes, a subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with the protein tyrosine kinase lck through its cytoplasmic tail. Binding of RM4-4 does not block binding of the CD4 monoclonal antibodies RM4-5 or GK1.5.Applications Reported: This RM4-4 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RM4-4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].
| 数据 |
| CD4 Antibody (61-0043-80) in FlowC57BL/6 mouse splenocytes were stained with CD3 Monoclonal Antibody, FITC (Product # 11-0031-82) and 0.125 µg of Rat IgG2b kappa Isotype Control, PE-eFluor 610 (Product # 61-4031-80) (left) or 0.125 µg of CD4 Monoclonal Antibody, PE-eFluor 610 (right). Cells in the lymphocyte gate were used for analysis. |
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