CD4 Monoclonal Antibody (RM4-5), Biotin,

货号：13-0042-82,13-0042-85,13-0042-86

规格：100 μg,500 ug,1 mg

价格：1011,2323,3949

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RM4-5

荧光染料：Biotin

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Biotin
浓度：	0.5 mg/mL	用法：	0.25 µg/test(Flow)

产品详细信息Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis.Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

数据

CD4 Antibody (13-0042-82) in FlowStaining of C57BL/6 splenocytes with 0.125 µg of Rat IgG2a K Isotype Control Biotin (Product # 13-4321-82) (open histogram) or 0.125 µg of Anti-Mouse CD4 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis. CD4 Antibody (13-0042-82)Frontiers in immunology 2020 -Figure 4 Ca V 1.4 deficiency results in a memory T cell phenotype. Splenocytes of WT ( n = 15 or n = 22) and KO ( n = 18 or n = 26) mice were stained with antibodies against CD3, CD4, CD8, CD62L, IL7R, and CD44 and analyzed by flow cytometry. The different population frequencies shown in boxplots are classified as CD62L+ IL-7R+ CD44- (naive), CD62L+ IL-7R+ CD44+ (T CM ), CD62L- IL-7R+ (T EM ), CD62L- IL-7R- (end-stage T Eff ). Boxplots show four pooled experiments * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

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参考文献

1. Cancer prevention research (Philadelphia, Pa.)PD-1 Blockade Prevents the Development and Progression of Carcinogen-Induced Oral Premalignant Lesions.2. Nature communicationsdNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.3. PloS oneCharacterization of two distinct lymphoproliferative diseases caused by ectopic expression of the Notch ligand DLL4 on T cells.4. Frontiers in immunologyMutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.5. Frontiers in neurologyIL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.

技术参数

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