CD4 Monoclonal Antibody (RM4-5), Super Bright 436,

货号：62-0042-80,62-0042-82

规格：25 ug ,100 μg

价格：1299,3050

产品类型：流式抗体

品牌：eBioscience

抗原：CD4

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RM4-5

荧光染料：Super Bright 436

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 436
浓度：	0.2 mg/mL	用法：	0.5 µg/test(Flow)

产品详细信息Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.Applications Reported: This RM4-5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RM4-5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.Excitation: 405 nm; Emission: 436 nm; Laser: Violet LaserSuper Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.靶标信息The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

数据

CD4 Antibody (62-0042-80) in FlowBALB/c mouse splenocytes were stained with CD3e Monoclonal Antibody, APC (Product # 17-0031-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (left) or 0.25 µg of CD4 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.

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