CD3e Monoclonal Antibody (eBio500A2 (500A2)), Alexa Fluor 700, eBioscience™

货号：56-0033-82

规格：100 µg

价格：2019

产品类型：流式抗体

品牌：eBioscience

抗原：CD3e

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

克隆号：eBio500A2 (500A2)

荧光染料：Alexa Fluor 700

类型:	一抗	同型对照：	IgG
浓度：	0.2 mg/mL	用法：	0.5 µg/test（Flow）

产品详细信息Description: The eBio500A2 monoclonal antibody reacts with the 25 kD epsilon subunit of the mouse CD3 complex. CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, as well as NKT cells. Binding of eBio500A2 to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. The 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.Applications Reported: This eBio500A2 (500A2) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio500A2 (500A2) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 nm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome.Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components havelong cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.仅用于科研。不用于诊断过程。

数据

CD3e Antibody (56-0033-82) in FlowStaining of BALB/c splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193-82) and staining buffer (autofluorescence) (left) or 0.25 µg of Anti-Mouse CD3e Alexa Fluor® 700 (right). Cells in the lymphocyte gate were used for analysis.

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文献：

1. Immune networkHerpes Zoster DNA Vaccines with IL-7 and IL-33 Molecular Adjuvants Elicit Protective T Cell Immunity."56-0033 was used in Flow cytometry/Cell sorting to evaluate the T cell immunogenicity of Herpes Zoster DNA vaccines."AuthorsKim AR,Park J,Kim JH,Kwak JE,Cho Y,Lee H,Jeong M,Park SH,Shin ECYear2018SpeciesMouse 2. Nature communicationsγδ T cells control humoral immune response by inducing T follicular helper cell differentiation."56-0033 was used in Flow cytometry/Cell sorting to demonstrate that complete Freund's adjuvant immunisation induces a subpopulation of CXCR5-expressing γδ T cells in the draining lymph nodes."AuthorsRezende RM,Lanser AJ,Rubino S,Kuhn C,Skillin N,Moreira TG,Liu S,Gabriely G,David BA,Menezes GB,Weiner HLYear2018SpeciesMouse 3. PloS oneCytokine and hormonal regulation of bone marrow immune cell Wnt10b expression."56-0033 was used in Flow cytometry/Cell sorting to determine the potential regulation of Wnt10b by estrogen and TNFα, showing regulation of expression by cytokines and hormones."AuthorsCollins FL,Rios-Arce ND,McCabe LR,Parameswaran NYear2017SpeciesMouse 4. PloS oneExpression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential."56-0033 was used in Flow cytometry/Cell sorting to find markers associated with non-conventional T cell subsets, including γδ T cells and invariant natural killer T cells."AuthorsQualai J,Li LX,Cantero J,Tarrats A,Fernández MA,Sumoy L,Rodolosse A,McSorley SJ,Genescà MYear2017SpeciesMouse 5. Journal of immunology (Baltimore, Md. : 1950)Selective Targeting of a Disease-Related Conformational Isoform of Macrophage Migration Inhibitory Factor Ameliorates Inflammatory Conditions."56-0033 was used in Flow cytometry/Cell sorting to demonstrate that oxMIF represents the disease-related isoform of MIF and is therefore a new diagnostic marker for inflammation."AuthorsThiele M,Kerschbaumer RJ,Tam FW,Völkel D,Douillard P,Schinagl A,Kühnel H,Smith J,McDaid JP,Bhangal G,Yu MC,Pusey CD,Cook HT,Kovarik J,Magelky E,Bhan A,Rieger M,Mudde GC,Ehrlich H,Jilma B,Tilg H,Moschen A,Terhorst C,Scheiflinger F

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